Optimization of fermentation medium for spore production of Paenibacillus polymyxa IN937a and its antifungal activity

In this study, we optimized the fermentation medium for spore production of Paenibacillus polymyxa IN937a. Seven factors including molasses, glucose, magnesium sulfate, potassium pyrophosphate, yeast extract, zinc and ammonium sulfate are selected as basis screening affecting P. IN937a by Plackett-Burman experiment. Based on analysis matrix, result showed that were three main impact (P < 0.05), which affected yield spores. Then, optimum combination was subsequently response surface methodology (RSM) using Box-Behnken design to increase in fermentation. The obtained results RSM predicted maximum density 6.606×109 spore/mL after 48 hours experiment when appropriate included extract 14.44 g/L, molasses 19.14 0.20 g/L. addition, antifungal activity also tested study. preliminary vitro indicated had a good inhibition growth two phytopathogenic fungal strains Sclerotium rolfsii Rhizoctonia solani. These could be used further research pilot scale obtain optimal number spores use development biological crop protection products.